PDBe 1ns0

X-ray diffraction
1.85Å resolution

Crystal structure of galactose mutarotase from Lactococcus lactis mutant E304Q complexed with galactose

Released:
Source organism: Lactococcus lactis
Primary publication:
The catalytic mechanism of galactose mutarotase.
Protein Sci. 12 1051-9 (2003)
PMID: 12717027

Function and Biology Details

Reaction catalysed:
Alpha-D-glucose = beta-D-glucose. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldose 1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.62 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZB17 (Residues: 1-339; Coverage: 100%)
Gene name: galM
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 44.8Å b: 76.2Å c: 211.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.21
Expression system: Escherichia coli