PDBe 1nek

X-ray diffraction
2.6Å resolution

Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero 12-mer
hetero octamer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase flavoprotein subunit Chain: A
Molecule details ›
Chain: A
Length: 588 amino acids
Theoretical weight: 64.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC41 (Residues: 1-588; Coverage: 100%)
Gene names: JW0713, b0723, sdhA
Sequence domains:
Structure domains:
Succinate dehydrogenase iron-sulfur subunit Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 26.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07014 (Residues: 1-238; Coverage: 100%)
Gene names: JW0714, b0724, sdhB
Sequence domains:
Structure domains:
Succinate dehydrogenase cytochrome b556 subunit Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69054 (Residues: 1-129; Coverage: 100%)
Gene names: JW0711, b0721, cybA, sdhC
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Succinate dehydrogenase hydrophobic membrane anchor subunit Chain: D
Molecule details ›
Chain: D
Length: 115 amino acids
Theoretical weight: 12.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC44 (Residues: 1-115; Coverage: 100%)
Gene names: JW0712, b0722, sdhD
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand UQ2 1 x UQ2

Cofactor: Ligand HEM 1 x HEM

Cofactor: Ligand FAD 1 x FAD

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 138.8Å b: 138.8Å c: 521.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.289
Expression system: Escherichia coli