PDBe 1n4p

X-ray diffraction
2.65Å resolution

Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate

Released:
Source organisms:
Primary publication:
Structure of mammalian protein geranylgeranyltransferase type-I.
EMBO J. 22 5963-74 (2003)
PMID: 14609943

Function and Biology Details

Reactions catalysed:
Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 377 amino acids
Theoretical weight: 44.1 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q04631 (Residues: 1-377; Coverage: 100%)
Gene name: Fnta
Sequence domains: Protein prenyltransferase alpha subunit repeat
Structure domains: Protein prenylyltransferase
Geranylgeranyl transferase type-1 subunit beta Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 377 amino acids
Theoretical weight: 42.47 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53610 (Residues: 1-377; Coverage: 100%)
Gene name: Pggt1b
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase
GTPase KRas, N-terminally processed Chains: M, N
Molecule details ›
Chains: M, N
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01116 (Residues: 185-185, 186-189; Coverage: 3%)
  • Best match: P01116-2 (Residues: 178-188)
Gene names: KRAS, KRAS2, RASK2

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 272.338Å b: 271.566Å c: 185.425Å
α: 90° β: 131.56° γ: 90°
R-values:
R R work R free
0.204 0.204 0.228
Expression systems:
  • Spodoptera frugiperda
  • Not provided