PDBe 1mzj

X-ray diffraction
2.1Å resolution

Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 35.43 KDa
Source organism: Streptomyces sp. R1128
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9F6D4 (Residues: 1-339; Coverage: 100%)
Gene names: fabH, zhuH
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P31
Unit cell:
a: 96Å b: 96Å c: 72.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.251
Expression system: Escherichia coli BL21(DE3)