PDBe 1mto

X-ray diffraction
3.2Å resolution

Crystal structure of a Phosphofructokinase mutant from Bacillus stearothermophilus bound with fructose-6-phosphate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 319 amino acids
Theoretical weight: 34.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00512 (Residues: 1-319; Coverage: 100%)
Gene names: pfk, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 110.682Å b: 106.872Å c: 119.588Å
α: 90° β: 113.98° γ: 90°
R-values:
R R work R free
0.189 0.18 0.28
Expression system: Escherichia coli