PDBe 1msw

X-ray diffraction
2.1Å resolution

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chain: D
Molecule details ›
Chain: D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
Template DNA Chain: T
Molecule details ›
Chain: T
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Enterobacteria phage T7
Expression system: Not provided
Non-Template DNA Chain: N
Molecule details ›
Chain: N
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Enterobacteria phage T7
Expression system: Not provided
Messenger RNA Chain: R
Molecule details ›
Chain: R
Length: 10 nucleotides
Theoretical weight: 3.24 KDa
Source organism: Enterobacteria phage T7
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 142.91Å b: 145.46Å c: 145.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.275
Expression systems:
  • Escherichia coli BL21
  • Not provided