PDBe 1mhd

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mothers against decapentaplegic homolog 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84022 (Residues: 1-132; Coverage: 31%)
Gene names: MADH3, SMAD3
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
DNA Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 45.6Å b: 60.4Å c: 71.6Å
α: 90° β: 102° γ: 90°
R-values:
R R work R free
0.212 0.212 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided