PDBe 1mbm

X-ray diffraction
2Å resolution

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C-like serine proteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 198 amino acids
Theoretical weight: 20.38 KDa
Source organism: Equine arteritis virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19811 (Residues: 1071-1268; Coverage: 6%)
Gene names: 1a-1b, rep
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 56.612Å b: 60.326Å c: 62.637Å
α: 74.08° β: 63.3° γ: 66.18°
R-values:
R R work R free
0.208 0.204 0.261
Expression system: Escherichia coli