› 1m7w

X-ray diffraction
2.8Å resolution

HNF4a ligand binding domain with bound fatty acid

Released:
DOI: 10.2210/pdb1m7w/pdb
PDB entry 1m7w coloured by chain, front view.
PDB entry 1m7w coloured by chain, side view.
PDB entry 1m7w coloured by chain, top view.
Source organism: Rattus rattus
Primary publication:
Crystal structure of the HNF4 alpha ligand binding domain in complex with endogenous fatty acid ligand.
Dhe-Paganon S, Duda K, Iwamoto M, Chi YI, Shoelson SE
J Biol Chem 277 37973-6 (2002)
PMID: 12193589

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hepatocyte nuclear factor 4-alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 250 amino acids
Theoretical weight: 28.12 KDa
Source organism: Rattus rattus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22449 (Residues: 142-391; Coverage: 53%)
Gene names: Hnf-4, Hnf4, Hnf4a, Nr2a1, Tcf14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand DAO 4 x DAO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 102.288Å b: 102.288Å c: 227.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

55 review citations

Nuclear receptors--a perspective from Drosophila.
King-Jones et al. (2005)
54 more

6 mentions without citation

Protein evolution by molecular tinkering: diversification of the nuclear receptor superfamily from a ligand-dependent ancestor.
Bridgham et al. (2010)
5 more