PDBe 1m5n

X-ray diffraction
2.9Å resolution

Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chain: S
Molecule details ›
Chain: S
Length: 485 amino acids
Theoretical weight: 53.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14974 (Residues: 1-485; Coverage: 55%)
Gene names: KPNB1, NTF97
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
PTHrP[38-94] Chain: Q
Molecule details ›
Chain: Q
Length: 28 amino acids
Theoretical weight: 3.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P12272 (Residues: 103-130; Coverage: 18%)
Gene names: PTHLH, PTHRP

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 92.792Å b: 105.447Å c: 146.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.297
Expression systems:
  • Escherichia coli BL21
  • Not provided