PDBe 1lvm

X-ray diffraction
1.8Å resolution

CATALYTICALLY ACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH PRODUCT

Released:

Function and Biology Details

Reactions catalysed:
Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
hetero pentamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear inclusion protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.15 KDa
Source organism: Tobacco etch virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04517 (Residues: 2038-2258; Coverage: 7%)
Structure domains: Trypsin-like serine proteases
Capsid protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.08 KDa
Source organism: Tobacco etch virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04517 (Residues: 2785-2794; Coverage: 0%)
Nuclear inclusion protein A Chain: E
Molecule details ›
Chain: E
Length: 7 amino acids
Theoretical weight: 806 Da
Source organism: Tobacco etch virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04517 (Residues: 2267-2273; Coverage: 0%)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P43212
Unit cell:
a: 75.505Å b: 75.505Å c: 183.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 0.23
Expression system: Escherichia coli