PDBe 1lmw

X-ray diffraction
2.5Å resolution

LMW U-PA Structure complexed with EGRCMK (GLU-GLY-ARG Chloromethyl Ketone)

Released:

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator short chain A Chains: A, C
Molecule details ›
Chains: A, C
Length: 23 amino acids
Theoretical weight: 2.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P00749 (Residues: 156-178; Coverage: 6%)
Gene name: PLAU
Urokinase-type plasminogen activator chain B Chains: B, D
Molecule details ›
Chains: B, D
Length: 253 amino acids
Theoretical weight: 28.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: R3
Unit cell:
a: 176.7Å b: 176.7Å c: 54.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 not available
Expression system: Escherichia coli