PDBe 1lm8

X-ray diffraction
1.85Å resolution

Structure of a HIF-1a-pVHL-ElonginB-ElonginC Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
von Hippel-Lindau disease tumor suppressor Chain: V
Molecule details ›
Chain: V
Length: 160 amino acids
Theoretical weight: 18.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P40337 (Residues: 54-213; Coverage: 75%)
Gene name: VHL
Sequence domains:
Structure domains:
Hypoxia-inducible factor 1-alpha Chain: H
Molecule details ›
Chain: H
Length: 20 amino acids
Theoretical weight: 2.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW Q16665 (Residues: 556-575; Coverage: 2%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P43212
Unit cell:
a: 59.5Å b: 59.5Å c: 245.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.236
Expression systems:
  • Escherichia coli
  • Not provided