PDBe 1l8c

Solution NMR

STRUCTURAL BASIS FOR HIF-1ALPHA/CBP RECOGNITION IN THE CELLULAR HYPOXIC RESPONSE

Released:
Source organisms:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CREB-binding protein Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P45481 (Residues: 345-439; Coverage: 4%)
Gene names: Cbp, Crebbp
Sequence domains: TAZ zinc finger
Structure domains: TAZ domain
Hypoxia-inducible factor 1-alpha Chain: B
Molecule details ›
Chain: B
Length: 51 amino acids
Theoretical weight: 5.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q16665 (Residues: 776-826; Coverage: 6%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8
Sequence domains: HIF-1 alpha C terminal transactivation domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Chemical shifts: BMR5327  
Expression system: Escherichia coli