PDBe 1kzy

X-ray diffraction
2.5Å resolution

Crystal Structure of the 53bp1 BRCT Region Complexed to Tumor Suppressor P53

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 21.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 95-289; Coverage: 50%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
TP53-binding protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 259 amino acids
Theoretical weight: 29.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12888 (Residues: 1714-1972; Coverage: 13%)
Gene name: TP53BP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 73.132Å b: 94.976Å c: 133.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.256
Expression system: Escherichia coli BL21(DE3)