PDBe 1kiu

X-ray diffraction
3Å resolution

FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type 1 fimbrin D-mannose specific adhesin Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 279 amino acids
Theoretical weight: 29.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08191 (Residues: 22-300; Coverage: 100%)
Gene names: JW4283, b4320, fimH
Sequence domains:
Structure domains: Immunoglobulin-like
Chaperone protein FimC Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 205 amino acids
Theoretical weight: 22.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 37-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 138.349Å b: 138.334Å c: 213.212Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.236 0.236 0.278
Expression system: Escherichia coli