PDBe 1kb6

X-ray diffraction
2.7Å resolution

Crystal Structure of VDR DNA-binding Domain Bound to Rat Osteocalcin (OC) Response Element

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Vitamin D3 receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P11473 (Residues: 16-125)
Gene names: NR1I1, VDR
5'-D(*CP*AP*CP*GP*GP*GP*TP*GP*AP*AP*TP*GP*AP*GP*GP*AP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.61 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*GP*TP*CP*CP*TP*CP*AP*TP*TP*CP*AP*CP*CP*CP*GP*TP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.42 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 61.15Å b: 61.15Å c: 241.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.275
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided