PDBe 1k5m

X-ray diffraction
2.7Å resolution

Crystal Structure of a Human Rhinovirus Type 14:Human Immunodeficiency Virus Type 1 V3 Loop Chimeric Virus MN-III-2

Released:

Function and Biology Details

Reactions catalysed:
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero 240-mer (preferred)
hetero tetramer
hetero 20-mer
hetero 24-mer
hetero 60-mer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.56 KDa
Source organism: Rhinovirus B14
Expression system: Homo sapiens
UniProt:
  • Canonical: P03303 (Residues: 568-856; Coverage: 13%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2; Surface protein gp120 Chain: B
Molecule details ›
Chain: B
Length: 277 amino acids
Theoretical weight: 30.1 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P05877 (Residues: 314-325; Coverage: 2%)
  • Canonical: P03303 (Residues: 70-228, 229-331; Coverage: 12%)
  • nullnull
Gene name: env
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 236 amino acids
Theoretical weight: 26.24 KDa
Source organism: Rhinovirus B14
Expression system: Homo sapiens
UniProt:
  • Canonical: P03303 (Residues: 332-567; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 68 amino acids
Theoretical weight: 7.18 KDa
Source organism: Rhinovirus B14
Expression system: Homo sapiens
UniProt:
  • Canonical: P03303 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Picornavirus coat protein VP4

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, CHESS BEAMLINE F1
Spacegroup: I222
Unit cell:
a: 318.9Å b: 349.3Å c: 368.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 not available
Expression system: Homo sapiens