› 1jpn

X-ray diffraction
1.9Å resolution

GMPPNP Complex of SRP GTPase NG Domain

Released:
DOI: 10.2210/pdb1jpn/pdb
PDB entry 1jpn coloured by chain, front view.
PDB entry 1jpn coloured by chain, side view.
PDB entry 1jpn coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase.
Padmanabhan S, Freymann DM
Structure 9 859-67 (2001)
PMID: 11566135

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.47 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GNP 2 x GNP
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 108.815Å b: 54.531Å c: 99.078Å
α: 90° β: 97.42° γ: 90°
R-values:
R R work R free
0.19 0.19 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
6 more

5 mentions without citation

The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase.
Padmanabhan et al. (2001)
4 more