PDBe 1jmo

X-ray diffraction
2.2Å resolution

Crystal Structure of the Heparin Cofactor II-S195A Thrombin Complex

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 48 amino acids
Theoretical weight: 5.48 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 315-362; Coverage: 8%)
Gene name: F2
Structure domains: Thrombin light chain domain
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 29.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 363-622; Coverage: 44%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Heparin cofactor 2 Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 55.19 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P05546 (Residues: 20-499; Coverage: 100%)
Gene names: HCF2, SERPIND1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P61
Unit cell:
a: 152.31Å b: 152.31Å c: 126.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.211
Expression system: Cricetulus griseus