PDBe 1jl1

X-ray diffraction
1.3Å resolution

D10A E. coli ribonuclease HI

Released:
Source organism: Escherichia coli
Primary publication:
Native-state energetics of a thermostabilized variant of ribonuclease HI.
J. Mol. Biol. 314 863-71 (2001)
PMID: 11734003

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-155; Coverage: 100%)
Gene names: JW0204, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 37.016Å b: 40.874Å c: 85.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.263
Expression system: Escherichia coli