PDBe 1jkg

X-ray diffraction
1.9Å resolution

Structural basis for the recognition of a nucleoporin FG-repeat by the NTF2-like domain of TAP-p15 mRNA nuclear export factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NTF2-related export protein 1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UKK6 (Residues: 1-140; Coverage: 100%)
Gene name: NXT1
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Nuclear RNA export factor 1 Chain: B
Molecule details ›
Chain: B
Length: 250 amino acids
Theoretical weight: 27.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBU9 (Residues: 371-619; Coverage: 40%)
Gene names: NXF1, TAP
Sequence domains:
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1, ELETTRA BEAMLINE 5.2R
Spacegroup: P6522
Unit cell:
a: 104.34Å b: 104.34Å c: 170.344Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.225
Expression system: Escherichia coli BL21(DE3)