PDBe 1jbu

X-ray diffraction
2Å resolution

Coagulation Factor VII Zymogen (EGF2/Protease) in Complex with Inhibitory Exosite Peptide A-183

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 63 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08709 (Residues: 150-212; Coverage: 14%)
Gene name: F7
Structure domains: Laminin
Peptide exosite inhibitor A-183 Chain: X
Molecule details ›
Chain: X
Length: 15 amino acids
Theoretical weight: 2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 67.44Å b: 84.51Å c: 84.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.204 0.227
Expression systems:
  • Trichoplusia ni
  • Escherichia coli