PDBe 1iy9

X-ray diffraction
2.3Å resolution

Crystal structure of spermidine synthase

Released:
Source organism: Bacillus subtilis
Entry authors: Tan AY, Smith PC, Shen J, Xiao R, Acton T, Rost B, Montelione G, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed:
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 31.24 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P70998 (Residues: 2-276; Coverage: 100%)
Gene names: BSU37500, speE, ywhF
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 96.1Å b: 105.8Å c: 121.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.254
Expression system: Escherichia coli