› 1ivy

X-ray diffraction
2.2Å resolution

PHYSIOLOGICAL DIMER HPP PRECURSOR

Released:
DOI: 10.2210/pdb1ivy/pdb
PDB entry 1ivy coloured by chain, front view.
PDB entry 1ivy coloured by chain, side view.
PDB entry 1ivy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism.
Rudenko G, Bonten E, d'Azzo A, Hol WG
Structure 3 1249-59 (1995)
PMID: 8591035

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid with broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lysosomal protective protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 51.49 KDa
Source organism: Homo sapiens
Expression system: SF21 INSECT CELLS
UniProt:
  • Canonical: P10619 (Residues: 29-480; Coverage: 100%)
Gene names: CTSA, PPGB
Sequence domains: Serine carboxypeptidase
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 115.04Å b: 148.11Å c: 80.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.268
Expression system: SF21 INSECT CELLS

Quick links

Citations

6 review citations

The metabolic serine hydrolases and their functions in mammalian physiology and disease.
Long et al. (2011)
5 more

14 mentions without citation

Heterodimerization of the sialidase NEU1 with the chaperone protective protein/cathepsin A prevents its premature oligomerization.
Bonten et al. (2009)
13 more