PDBe 1i4t

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE ANALYSIS OF RAC1-GMPPNP IN COMPLEX WITH ARFAPTIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Arfaptin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53365 (Residues: 118-341; Coverage: 66%)
Gene names: ARFIP2, POR1
Structure domains: Arfaptin homology (AH) domain/BAR domain
Ras-related C3 botulinum toxin substrate 1 Chain: D
Molecule details ›
Chain: D
Length: 192 amino acids
Theoretical weight: 21.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-192; Coverage: 100%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41
Unit cell:
a: 112.746Å b: 112.746Å c: 68.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.259 0.304
Expression system: Escherichia coli