› 1huq

X-ray diffraction
1.8Å resolution

1.8A CRYSTAL STRUCTURE OF THE MONOMERIC GTPASE RAB5C (MOUSE)

Released:
DOI: 10.2210/pdb1huq/pdb
PDB entry 1huq coloured by chain, front view.
PDB entry 1huq coloured by chain, side view.
PDB entry 1huq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural plasticity of an invariant hydrophobic triad in the switch regions of Rab GTPases is a determinant of effector recognition.
Merithew E, Hatherly S, Dumas JJ, Lawe DC, Heller-Harrison R, Lambright DG
J Biol Chem 276 13982-8 (2001)
PMID: 11278565

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-5C Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35278 (Residues: 19-182; Coverage: 76%)
Gene name: Rab5c
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 35.85Å b: 64Å c: 65.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Rabs and their effectors: achieving specificity in membrane traffic.
Grosshans et al. (2006)
5 more

4 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
3 more