PDBe 1hqr

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF A SUPERANTIGEN BOUND TO THE HIGH-AFFINITY, ZINC-DEPENDENT SITE ON MHC CLASS II

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class II histocompatibility antigen, DR beta 5 chain Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 22.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q30154 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB5
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
Myelin basic protein Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02686 (Residues: 221-233; Coverage: 4%)
Gene name: MBP
Exotoxin type C Chain: D
Molecule details ›
Chain: D
Length: 208 amino acids
Theoretical weight: 24.38 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NKX2 (Residues: 28-235; Coverage: 100%)
Gene names: speC, spyM18_0778
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 61.827Å b: 111.95Å c: 216.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.288
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)