PDBe 1hh4

X-ray diffraction
2.7Å resolution

Rac1-RhoGDI complex involved in NADPH oxidase activation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63000 (Residues: 2-192; Coverage: 100%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rho GDP-dissociation inhibitor 1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 204 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P52565 (Residues: 1-204; Coverage: 100%)
Gene names: ARHGDIA, GDIA1
Sequence domains: RHO protein GDP dissociation inhibitor
Structure domains: Coagulation Factor XIII, subunit A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 154.7Å b: 88.7Å c: 62.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.256 0.28
Expression system: Spodoptera frugiperda