PDBe 1he1

X-ray diffraction
2Å resolution

Crystal structure of the complex between the GAP domain of the Pseudomonas aeruginosa ExoS toxin and human Rac

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exoenzyme S Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.26 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51451 (Residues: 96-229; Coverage: 30%)
Gene name: exoS
Sequence domains: Yersinia virulence determinant (YopE)
Structure domains: Virulence factor YopE uncharacterised domain
Ras-related C3 botulinum toxin substrate 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 176 amino acids
Theoretical weight: 19.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 2-176; Coverage: 91%)
Gene names: MIG5, RAC1, TC25
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 46.025Å b: 125.087Å c: 136.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.224
Expression system: Escherichia coli