PDBe 1h9d

X-ray diffraction
2.6Å resolution

Aml1/cbf-beta/dna complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Runt-related transcription factor 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 134 amino acids
Theoretical weight: 14.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01196 (Residues: 50-183; Coverage: 30%)
Gene names: AML1, CBFA2, RUNX1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like
Core-binding factor subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 15.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13951 (Residues: 2-135; Coverage: 74%)
Gene name: CBFB
Structure domains: Core binding factor, beta subunit
DNA (5'-(*GP*TP*TP*GP*CP*GP*GP*TP*TP*G)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
DNA (5'-(*CP*AP*AP*CP*CP*GP*CP*AP*AP*C)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 10 nucleotides
Theoretical weight: 2.98 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P43212
Unit cell:
a: 115.033Å b: 115.033Å c: 133.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.271
Expression system: Escherichia coli BL21(DE3)