PDBe 1gzh

X-ray diffraction
2.6Å resolution

Crystal structure of the BRCT domains of human 53BP1 bound to the p53 tumor supressor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 95-292; Coverage: 50%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
TP53-binding protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 249 amino acids
Theoretical weight: 28.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12888 (Residues: 1724-1972; Coverage: 13%)
Gene name: TP53BP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
Cellular tumor antigen p53 Chain: C
Molecule details ›
Chain: C
Length: 198 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 95-292; Coverage: 50%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 71.52Å b: 94.57Å c: 136.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.288
Expression system: Escherichia coli