PDBe 1gmp

X-ray diffraction
1.7Å resolution

COMPLEX OF RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS WITH 2'-GMP AT 1.7 ANGSTROMS RESOLUTION

Released:
Source organism: Kitasatospora aureofaciens
Primary publication:
Complex of ribonuclease from Streptomyces aureofaciens with 2'-GMP at 1.7 A resolution.
Acta Crystallogr. D Biol. Crystallogr. 49 257-71 (1993)
PMID: 15299531

Function and Biology Details

Reaction catalysed:
Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.58 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Not provided
UniProt:
  • Canonical: P05798 (Residues: 1-96; Coverage: 100%)
Gene name: rnaSA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.7Å b: 78.8Å c: 39.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 not available not available
Expression system: Not provided