PDBe 1gh6

X-ray diffraction
3.2Å resolution

RETINOBLASTOMA POCKET COMPLEXED WITH SV40 LARGE T ANTIGEN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Large T antigen Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.44 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03070 (Residues: 7-117; Coverage: 16%)
Structure domains: Helix Hairpins
Retinoblastoma-associated protein Chain: B
Molecule details ›
Chain: B
Length: 333 amino acids
Theoretical weight: 39.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06400 (Residues: 379-577, 645-772; Coverage: 35%)
Gene name: RB1
Sequence domains: Retinoblastoma-associated protein B domain
Structure domains: Cyclin A; domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4
Unit cell:
a: 127.13Å b: 127.13Å c: 96.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.314
Expression system: Escherichia coli BL21