PDBe 1g4u

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP BOUND TO RAC1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Secreted effector protein SptP Chain: S
Molecule details ›
Chain: S
Length: 383 amino acids
Theoretical weight: 42.13 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: NEW P74873 (Residues: 161-543; Coverage: 71%)
Gene names: STM2878, sptP
Sequence domains:
Structure domains:
Ras-related C3 botulinum toxin substrate 1 Chain: R
Molecule details ›
Chain: R
Length: 184 amino acids
Theoretical weight: 20.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: NEW P63000 (Residues: 1-184; Coverage: 96%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 56.722Å b: 58.26Å c: 60.324Å
α: 70.03° β: 69.51° γ: 65.03°
R-values:
R R work R free
0.204 0.204 0.258
Expression system: Escherichia coli BL21