PDBe 1fqj

X-ray diffraction
2.02Å resolution

CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(t) subunit alpha-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 325 amino acids
Theoretical weight: 37.36 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 220-299; Coverage: 23%)
  • Canonical: P04695 (Residues: 26-215, 296-350; Coverage: 70%)
  • nullnull
Gene names: GNAT1, Gnai-1, Gnai1
Structure domains:
Regulator of G-protein signaling 9 Chains: B, E
Molecule details ›
Chains: B, E
Length: 147 amino acids
Theoretical weight: 17.42 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: O46469 (Residues: 276-422; Coverage: 30%)
Gene name: RGS9
Sequence domains: Regulator of G protein signaling domain
Structure domains:
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 42 amino acids
Theoretical weight: 4.56 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04972 (Residues: 46-87; Coverage: 48%)
Gene names: PDE6G, PDEG

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 91.609Å b: 115.937Å c: 134.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.265
Expression system: Escherichia coli