PDBe 1foe

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF RAC1 IN COMPLEX WITH THE GUANINE NUCLEOTIDE EXCHANGE REGION OF TIAM1

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T-lymphoma invasion and metastasis-inducing protein 1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 377 amino acids
Theoretical weight: 43.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60610 (Residues: 1030-1406; Coverage: 24%)
Gene names: Tiam-1, Tiam1
Sequence domains:
Structure domains:
Ras-related C3 botulinum toxin substrate 1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 177 amino acids
Theoretical weight: 19.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 186.95Å b: 149.27Å c: 149.21Å
α: 90° β: 121° γ: 90°
R-values:
R R work R free
0.262 0.262 0.293
Expression system: Escherichia coli