PDBe 1fka

X-ray diffraction
3.3Å resolution

STRUCTURE OF FUNCTIONALLY ACTIVATED SMALL RIBOSOMAL SUBUNIT AT 3.3 A RESOLUTION

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 20-mer (preferred)
Entry contents:
19 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (20 distinct):
30S RIBOSOMAL PROTEIN S2 Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 9.46 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S3 Chain: C
Molecule details ›
Chain: C
Length: 176 amino acids
Theoretical weight: 15 KDa
Source organism: Thermus thermophilus
30S ribosomal protein S4 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 24.37 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80373 (Residues: 1-209; Coverage: 100%)
Gene names: TTHA1665, rps4, rpsD
Sequence domains:
Structure domains:
30S ribosomal protein S5 Chain: E
Molecule details ›
Chain: E
Length: 162 amino acids
Theoretical weight: 17.58 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHQ5 (Residues: 1-162; Coverage: 100%)
Gene names: TTHA1675, rpsE
Sequence domains:
Structure domains:
30S ribosomal protein S6 Chain: F
Molecule details ›
Chain: F
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLP8 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA0245, rpsF
Sequence domains: Ribosomal protein S6
Structure domains: Alpha-Beta Plaits
30S ribosomal protein S7 Chain: G
Molecule details ›
Chain: G
Length: 151 amino acids
Theoretical weight: 17.34 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P17291 (Residues: 1-151; Coverage: 97%)
Gene names: TTHA1696, rps7, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Structure domains: Ribosomal protein S7 domain
30S ribosomal protein S8 Chain: H
Molecule details ›
Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: A0A0M9AFS9 (Residues: 1-138; Coverage: 100%)
Gene names: BVI061214_01034, rpsH
Sequence domains: Ribosomal protein S8
Structure domains:
30S RIBOSOMAL PROTEIN S9 Chain: I
Molecule details ›
Chain: I
Length: 89 amino acids
Theoretical weight: 7.59 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S10 Chain: J
Molecule details ›
Chain: J
Length: 71 amino acids
Theoretical weight: 6.06 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S11 Chain: K
Molecule details ›
Chain: K
Length: 70 amino acids
Theoretical weight: 5.98 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S12 Chain: L
Molecule details ›
Chain: L
Length: 103 amino acids
Theoretical weight: 8.78 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S13 Chain: M
Molecule details ›
Chain: M
Length: 77 amino acids
Theoretical weight: 6.57 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S14 Chain: N
Molecule details ›
Chain: N
Length: 26 amino acids
Theoretical weight: 2.23 KDa
Source organism: Thermus thermophilus
30S ribosomal protein S15 Chain: O
Molecule details ›
Chain: O
Length: 89 amino acids
Theoretical weight: 10.62 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Sequence domains: Ribosomal protein S15
Structure domains: S15/NS1, RNA-binding
30S RIBOSOMAL PROTEIN S16 Chain: P
Molecule details ›
Chain: P
Length: 73 amino acids
Theoretical weight: 6.23 KDa
Source organism: Thermus thermophilus
30S RIBOSOMAL PROTEIN S17 Chain: Q
Molecule details ›
Chain: Q
Length: 84 amino acids
Theoretical weight: 7.17 KDa
Source organism: Thermus thermophilus
30S ribosomal protein S18 Chain: R
Molecule details ›
Chain: R
Length: 88 amino acids
Theoretical weight: 10.24 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Sequence domains: Ribosomal protein S18
Structure domains: Ribosomal protein S18
30S ribosomal protein S19 Chain: S
Molecule details ›
Chain: S
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Sequence domains: Ribosomal protein S19
Structure domains: 30s Ribosomal Protein S19; Chain A
30S RIBOSOMAL PROTEIN S20 Chain: T
Molecule details ›
Chain: T
Length: 95 amino acids
Theoretical weight: 8.1 KDa
Source organism: Thermus thermophilus
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1518 nucleotides
Theoretical weight: 492.67 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 406.3Å b: 406.3Å c: 173.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.304 0.304 0.305