PDBe 1ffy

X-ray diffraction
2.2Å resolution

INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Isoleucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 917 amino acids
Theoretical weight: 105.06 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P41972 (Residues: 1-917; Coverage: 100%)
Gene name: ileS
Sequence domains:
Structure domains:
ISOLEUCYL-TRNA Chain: T
Molecule details ›
Chain: T
Length: 75 nucleotides
Theoretical weight: 24.24 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 71Å b: 100Å c: 186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.281
Expression systems:
  • Escherichia coli
  • Not provided