PDBe 1f45

X-ray diffraction
2.8Å resolution

HUMAN INTERLEUKIN-12

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-12 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 197 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P29459 (Residues: 23-219; Coverage: 100%)
Gene names: IL12A, NKSF1
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 112.9Å b: 154.2Å c: 101.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.284
Expression system: Cricetulus griseus