› 1egz

X-ray diffraction
2.3Å resolution

CELLULASE CEL5 FROM ERWINIA CHRYSANTHEMI, A FAMILY GH 5-2 ENZYME

Released:
DOI: 10.2210/pdb1egz/pdb
PDB entry 1egz coloured by chain, front view.
PDB entry 1egz coloured by chain, side view.
PDB entry 1egz coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Type II protein secretion in gram-negative pathogenic bacteria: the study of the structure/secretion relationships of the cellulase Cel5 (formerly EGZ) from Erwinia chrysanthemi.
Chapon V, Czjzek M, El Hassouni M, Py B, Juy M, Barras F
J Mol Biol 310 1055-66 (2001)
PMID: 11501995

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Z Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 291 amino acids
Theoretical weight: 32.13 KDa
Source organism: Dickeya chrysanthemi
UniProt:
  • Canonical: P07103 (Residues: 44-334; Coverage: 76%)
Gene names: Dda3937_02793, cel5, cel5Z, celZ
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 168.8Å b: 47.2Å c: 120.1Å
α: 90° β: 105° γ: 90°
R-values:
R R work R free
0.179 0.179 0.263

Quick links

Citations

8 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
7 more

7 mentions without citation

Kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the Antarctic bacterium Pseudoalteromonas haloplanktis.
Garsoux et al. (2004)
6 more