› 1eg2

X-ray diffraction
1.75Å resolution

CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)

Released:
DOI: 10.2210/pdb1eg2/pdb
PDB entry 1eg2 coloured by chain, front view.
PDB entry 1eg2 coloured by chain, side view.
PDB entry 1eg2 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.
Scavetta RD, Thomas CB, Walsh MA, Szegedi S, Joachimiak A, Gumport RI, Churchill ME
Nucleic Acids Res 28 3950-61 (2000)
PMID: 11024175

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-147066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M.RsrI Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 35.7 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P14751 (Residues: 1-319; Coverage: 100%)
Gene name: rsrIM
Sequence domains: DNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand MTA 1 x MTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 70.42Å b: 130.253Å c: 67.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.25
Expression system: Not provided

Quick links

Citations

14 review citations

Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.
Jeltsch A. (2002)
13 more

7 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
6 more