› 1e3j

X-ray diffraction
2.3Å resolution

Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly

Released:
DOI: 10.2210/pdb1e3j/pdb
PDB entry 1e3j coloured by chain, front view.
PDB entry 1e3j coloured by chain, side view.
PDB entry 1e3j coloured by chain, top view.
Source organism: Bemisia argentifolii
Primary publication:
Crystal structure of the NADP(H)-dependent ketose reductase from Bemisia argentifolii at 2.3 A resolution.
Banfield MJ, Salvucci ME, Baker EN, Smith CA
J. Mol. Biol. 306 239-50 (2001)
PMID: 11237597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorbitol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 38.21 KDa
Source organism: Bemisia argentifolii
Expression system: Escherichia coli
UniProt:
  • Canonical: O96496 (Residues: 1-352; Coverage: 100%)
Gene name: Kr
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand PO4 1 x PO4
Ligand BO3 1 x BO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 78.822Å b: 98.395Å c: 120.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.25
Expression system: Escherichia coli

Quick links

Citations

37 citation in other articles

Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc.
Watanabe et al. (2005)
36 more

12 mentions without citation

Amino acid empirical contact energy definitions for fold recognition in the space of contact maps.
Berrera et al. (2003)
11 more