PDBe 1doj

X-ray diffraction
1.7Å resolution

Crystal structure of human alpha-thrombin*RWJ-51438 complex at 1.7 A

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 328-622; Coverage: 49%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin variant-2 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.49 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P09945 (Residues: 62-72; Coverage: 17%)

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 62.982Å b: 117.521Å c: 47.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.217
Expression system: Not provided