PDBe 1dev

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF SMAD2 MH2 DOMAIN BOUND TO THE SMAD-BINDING DOMAIN OF SARA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 196 amino acids
Theoretical weight: 22.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15796 (Residues: 261-456; Coverage: 42%)
Gene names: MADH2, MADR2, SMAD2
Sequence domains: MH2 domain
Structure domains: Tumour Suppressor Smad4
Zinc finger FYVE domain-containing protein 9 Chains: B, D
Molecule details ›
Chains: B, D
Length: 41 amino acids
Theoretical weight: 4.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95405 (Residues: 771-811; Coverage: 3%)
Gene names: MADHIP, SARA, SMADIP, ZFYVE9
Sequence domains: Smad anchor for receptor activation (SARA)
Structure domains: Smad anchor for receptor activation, Smad-binding domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 138.5Å b: 138.5Å c: 55.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.276
Expression system: Escherichia coli