PDBe 1d01

X-ray diffraction
2Å resolution

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A HUMAN CD30 PEPTIDE

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo trimer
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TNF receptor-associated factor 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 168 amino acids
Theoretical weight: 19.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12933 (Residues: 334-501; Coverage: 34%)
Gene names: TRAF2, TRAP3
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Tumor necrosis factor receptor superfamily member 8 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 9 amino acids
Theoretical weight: 919 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P28908 (Residues: 576-583; Coverage: 1%)
Gene names: CD30, D1S166E, TNFRSF8

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 91.3Å b: 110.4Å c: 91.3Å
α: 90° β: 120° γ: 90°
R-values:
R R work R free
0.22 0.22 0.244
Expression systems:
  • Escherichia coli
  • Not provided