› 1cwq

X-ray diffraction
2.25Å resolution

M INTERMEDIATE STRUCTURE OF THE WILD TYPE BACTERIORHODOPSIN IN COMBINATION WITH THE GROUND STATE STRUCTURE

Released:
DOI: 10.2210/pdb1cwq/pdb
PDB entry 1cwq coloured by chain, front view.
PDB entry 1cwq coloured by chain, side view.
PDB entry 1cwq coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin.
Sass HJ, Büldt G, Gessenich R, Hehn D, Neff D, Schlesinger R, Berendzen J, Ormos P
Nature 406 649-53 (2000)
PMID: 10949308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.89 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-261; Coverage: 94%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

5 bound ligands:
Ligand RET 2 x RET
Ligand UND 8 x UND
Ligand OCT 16 x OCT
Ligand HEX 12 x HEX
Ligand TRD 6 x TRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P63
Unit cell:
a: 61.08Å b: 61.08Å c: 110.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.167 0.236
Expression system: Halobacterium salinarum

Quick links

Citations

27 review citations

Bacteriorhodopsin.
Lanyi JK. (2004)
26 more

21 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
20 more