PDBe 1cqi

X-ray diffraction
3.3Å resolution

Crystal Structure of the Complex of ADP and MG2+ with Dephosphorylated E. Coli Succinyl-CoA Synthetase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP-forming] subunit alpha Chains: A, D
Molecule details ›
Chains: A, D
Length: 286 amino acids
Theoretical weight: 29.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGE9 (Residues: 2-287; Coverage: 99%)
Gene names: JW0718, b0729, sucD
Sequence domains:
Structure domains:
Succinate--CoA ligase [ADP-forming] subunit beta Chains: B, E
Molecule details ›
Chains: B, E
Length: 385 amino acids
Theoretical weight: 41.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A836 (Residues: 1-385; Coverage: 99%)
Gene names: JW0717, b0728, sucC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P4322
Unit cell:
a: 99.86Å b: 99.86Å c: 407.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.247
Expression system: Escherichia coli