PDBe 1ckm

X-ray diffraction
2.5Å resolution

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP

Released:

Function and Biology Details

Reaction catalysed:
GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.88 KDa
Source organism: Paramecium bursaria Chlorella virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84424 (Residues: 1-330; Coverage: 100%)
Gene name: A103R
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: C2221
Unit cell:
a: 93.332Å b: 214.931Å c: 105.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.299
Expression system: Escherichia coli