PDBe 1ca1

X-ray diffraction
1.9Å resolution

ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS

Released:
Source organism: Clostridium perfringens
Primary publication:
Structure of the key toxin in gas gangrene.
Nat. Struct. Biol. 5 738-46 (1998)
PMID: 9699639

Function and Biology Details

Reaction catalysed:
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.62 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C216 (Residues: 29-398; Coverage: 100%)
Gene names: CPE0036, cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: C2221
Unit cell:
a: 61.3Å b: 177.3Å c: 79.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.254
Expression system: Escherichia coli